HORIBA
Jobin Yvon, the leader in fluorescence spectroscopy,
now offers the Protein 280 exT, a lifetime spectrofluorometer
that can measure
three fluorescence lifetimes in just a few minutes. Ideal
for those biologists and biochemists who need to test
multiple protein samples
fast, the Protein 280 exT uses Time-Correlated Single-Photon
Counting, a time-resolved fluorescence technique, to
record accurate, reproducible
lifetime data. Analytical software then calculates lifetimes
based on least-squares fitting algorithms. The system
uses the modular FluoroCube,
with a pulsed 280 nm UV NanoLED source. NanoLEDs are
miniature turn-key light-sources that generate short
optical pulses from <100 ps to
1 ns. The 280 nm LED matches the absorption maxima of
the naturally fluorescent amino acids tryptophan and
tyrosine. Thus, the Protein 280
exT is well-suited for examining biomarkers and studying
the dynamics of protein folding. Tryptophan fluorescence
is widely used as a probe
of protein structure and dynamics.
Pulsed light-source (gray) and response (black) of the sample, POPOP, showing the gradual decay of fluorescence intensity with time. A single-exponential fit (dotted red) gives a lifetime of 1.309 ± 0.003 ns.
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